Martyrdom
The sources relate that the rebellion of Metropolitan Dionysios the Philosopher in 1601 was suppressed harshly, and that Seraphim was accused of complicity in it. He was imprisoned and pressed repeatedly to convert to Islam, which he refused to do.
The tradition records that he endured grievous tortures, including the severing of his nose, and that he was finally impaled near a cypress tree in the vicinity of the marketplace of Phanarion, dying of his wounds. His death is dated to December 4, 1601.